产品名称
(Mouse) Lin28a Rabbit Polyclonal Antibody (C-term)
别名
Protein lin-28 homolog A, Lin-28A, Testis-expressed protein 17, Lin28a, Lin28, Tex17
Human Swissprot No.
Q8K3Y3
特异性
This Mouse Lin28a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 177-210 amino acids from the C-terminal region of Mouse Lin28a.
背景介绍
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, Lin28a down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'- GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
组织表达
Expressed in embryonic stem cells (ES cells), spermatagonia and testis. Expressed in numerous epithelial tissues including the epithelia of the small intestine, the intralobular duct epithelium of the mammary gland and the epithelia of Henle's loop in the kidney and in the collecting duct (at protein level). Also expressed in the myocardium and skeletal muscle (at protein level)
细胞定位
Cytoplasm. Rough endoplasmic reticulum. Cytoplasm, P-body {ECO:0000250|UniProtKB:Q9H9Z2}. Cytoplasm, Stress granule. Nucleus, nucleolus. Note=Predominantly cytoplasmic (PubMed:12798299). In the cytoplasm, localizes to peri-endoplasmic reticulum regions and detected in the microsomal fraction derived from rough endoplasmic reticulum (RER) following subcellular fractionation May be bound to the cytosolic surface of RER on which ER-associated mRNAs are translated (PubMed:23102813). Shuttle from the nucleus to the cytoplasm requires RNA-binding (PubMed:17473174). Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (PubMed:17473174).
