All lanes : Anti-Rps6ka1 Antibody (C-term) at 1:2000 dilution Lane 1: C6 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: L929 whole cell lysates Lane 4: mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.