BD-PB3235-EXOC3 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

EXOC3 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB3235

产品名称

EXOC3 Rabbit Polyclonal Antibody (C-term)

别名

Exocyst complex component 3, Exocyst complex component Sec6, EXOC3, SEC6, SEC6L1

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

O60645

特异性

This EXOC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 524-557 amino acids from the C-terminal region of human EXOC3.

稀释度

IHC-P~~1:100~500;WB~~1:2000

预测分子量

86kDa

同种型

Rabbit Ig

背景介绍

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

组织表达

Expressed in epididymis (at protein level).

细胞定位

Cytoplasm {ECO:0000250|UniProtKB:O54921}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O54921}. Cell projection, growth cone {ECO:0000250|UniProtKB:O54921}. Midbody. Golgi apparatus. Cell projection, neuron projection {ECO:0000250|UniProtKB:Q62825}. Note=Perinuclear in undifferentiated cells. Redistributes to growing neurites and growth cones during neuronal differentiation (By similarity). During mitosis, early recruitment to the midbody requires RALA, but not RALB, and EXOC2. In late stages of cytokinesis, localization to the midbody is RALB- dependent (PubMed:18756269). {ECO:0000250|UniProtKB:O54921, ECO:0000269|PubMed:18756269}

期货

现货

BD-PB3235 staining EXOC3 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-EXOC3 Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: human brain lysates Lane 3: Hela whole cell lysates Lane 4: U-2OS whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.