BD-PB3224-(Mouse) Med15 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

(Mouse) Med15 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB3224

产品名称

(Mouse) Med15 Rabbit Polyclonal Antibody (C-term)

别名

Mediator of RNA polymerase II transcription subunit 15, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, mPcqap, Med15, Pcqap

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

Q924H2

特异性

This mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.

稀释度

IHC-P~~1:100~500;WB~~1:2000

预测分子量

87kDa

同种型

Rabbit Ig

背景介绍

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).

细胞定位

Cytoplasm. Nucleus.

期货

现货

BD-PB3224 staining (Mouse) Med15 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-Med15 Antibody (C-term) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: mouse spleen lysates Lane 3: mouse thymus lysates Lane 4: rat lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.