BD-PB3221-(Mouse) Smad1 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

(Mouse) Smad1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB3221

产品名称

(Mouse) Smad1 Rabbit Polyclonal Antibody (Center)

别名

Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD family member 1, SMAD 1, Smad1, Smad1, Madh1, Madr1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

P70340

特异性

This Mouse Smad1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 154-187 amino acids from the Central region of Mouse Smad1.

稀释度

IHC-P~~1:100~500;WB~~1:1000

预测分子量

52kDa

同种型

Rabbit Ig

背景介绍

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) (By similarity). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene [removed]PubMed:15329343).

组织表达

Ubiquitous.

细胞定位

Cytoplasm. Nucleus. Note=In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. Colocalizes with LEMD3 at the nucleus inner membrane. Exported from the nucleus to the cytoplasm when dephosphorylated (PubMed:25755279) {ECO:0000250|UniProtKB:Q15797, ECO:0000269|PubMed:25755279}

期货

现货

BD-PB3221 staining (Mouse) Smad1 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-Smad1 Antibody (Center) at 1:1000 dilution + mouse skeletal muscle lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.