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DNASE1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号
BD-PB3185
产品名称
DNASE1 Rabbit Polyclonal Antibody (Center)
别名
Deoxyribonuclease-1, Deoxyribonuclease I, DNase I, Dornase alfa, DNASE1, DNL1, DRNI
类别
常规抗体
推荐应用
WB
反应种属
Human, Mouse
Human Swissprot No.
P24855
特异性
This DNASE1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 87-121 amino acids from the Central region of human DNASE1.
稀释度
IHC-P-Leica~~1:500;WB~~1:1000
预测分子量
31kDa
同种型
Rabbit Ig
背景介绍
Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).
组织表达
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva
细胞定位
Secreted. Zymogen granule. Nucleus envelope. Note=Secretory protein, stored in zymogen granules and found in the nuclear envelope
期货
现货

Immunohistochemical analysis of paraffin-embedded Human stomach tissue using BD-PB3185 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Anti-DNASE1 Antibody (Center) at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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