BD-PB3156-(Mouse) Pdx1 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

(Mouse) Pdx1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB3156

产品名称

(Mouse) Pdx1 Rabbit Polyclonal Antibody (Center)

别名

Pancreas/duodenum homeobox protein 1, Insulin promoter factor 1, IPF-1, Islet/duodenum homeobox 1, IDX-1, Somatostatin-transactivating factor 1, STF-1, Pdx1, Ipf1

类别

常规抗体

推荐应用

反应种属

Mouse

Human Swissprot No.

P52946

特异性

This Mouse Pdx1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 136-169 amino acids from the Central region of Mouse Pdx1.

稀释度

WB~~1:2000

预测分子量

31kDa

同种型

Rabbit Ig

背景介绍

Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.

组织表达

Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells)

细胞定位

Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:17052199}. Cytoplasm, cytosol

期货

现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with BD-PB3156 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).

Western blot analysis of lysate from mouse pancreas tissue lysate, using Pdx1 Antibody (Center). BD-PB3156 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.