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DLK1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB3085
产品名称
DLK1 Rabbit Polyclonal Antibody (C-term)
别名
Protein delta homolog 1, DLK-1, pG2, Fetal antigen 1, FA1, DLK1, DLK
类别
常规抗体
推荐应用
WB
反应种属
Human, Mouse
Human Swissprot No.
P80370
特异性
This DLK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 368-402 amino acids from the C-terminal region of human DLK1.
稀释度
WB~~1:1000
预测分子量
41kDa
同种型
Rabbit Ig
背景介绍
May have a role in neuroendocrine differentiation.
组织表达
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue
细胞定位
Membrane; Single-pass type I membrane protein. Cytoplasm {ECO:0000250|UniProtKB:O70534}
期货
现货

All lanes : Anti-DLK1 Antibody (C-term) at 1:1000 dilution Lane 1: human placenta lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. placenta section using DLK1 Antibody (C-term). BD-PB3085 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Flow cytometric analysis of HepG2 cells using DLK1 Antibody (C-term)(green) compared to an isotype control of mouse IgG2b(blue). BD-PB3085 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from human placenta, mouse skeletal muscle tissue, NCCIT cell line (from left to right), using DLK1 Antibody (C-term). BD-PB3085 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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