DLK1 Rabbit Polyclonal Antibody (C-term)
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All lanes : Anti-DLK1 Antibody (C-term) at 1:1000 dilution Lane 1: human placenta lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded H. placenta section using DLK1 Antibody (C-term). BD-PB3085 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using DLK1 Antibody (C-term)(green) compared to an isotype control of mouse IgG2b(blue). BD-PB3085 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from human placenta, mouse skeletal muscle tissue, NCCIT cell line (from left to right), using DLK1 Antibody (C-term). BD-PB3085 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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