BD-PB3045-(Mouse) Smarcc1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

(Mouse) Smarcc1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB3045

产品名称

(Mouse) Smarcc1 Rabbit Polyclonal Antibody (C-term)

别名

SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI3-related protein, BAF155, Smarcc1, Baf155, Srg3

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

P97496

特异性

This Mouse Smarcc1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 783-817 amino acids from the C-terminal region of Mouse Smarcc1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:25

预测分子量

123kDa

同种型

Rabbit Ig

背景介绍

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

组织表达

Highly expressed in adult brain, testis and thymus.

细胞定位

Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q92922}

期货

现货

All lanes : Anti-Smarcc1 Antibody (C-term) at 1:1000 dilution Lane 1: C2C12 whole cell lysates Lane 2: C6 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded M. testis section using (Mouse) Smarcc1 Antibody (C-term). BD-PB3045 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of Hela cells stained with (Mouse) Smarcc1 Antibody (C-term). BD-PB3045 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Western blot analysis of lysate from Hela cell line, using Smarcc1 Antibody (C-term). BD-PB3045 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.