BD-PB3045-(Mouse) Smarcc1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

(Mouse) Smarcc1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB3045

产品名称

(Mouse) Smarcc1 Rabbit Polyclonal Antibody (C-term)

别名

SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI3-related protein, BAF155, Smarcc1, Baf155, Srg3

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

P97496

特异性

This Mouse Smarcc1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 783-817 amino acids from the C-terminal region of Mouse Smarcc1.

稀释度

WB 1:1000

预测分子量

123kDa

同种型

Rabbit Ig

背景介绍

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). May stimulate the ATPase activity of the catalytic subunit of the complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

组织表达

Highly expressed in adult brain, testis and thymus.

细胞定位

Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q92922}

期货

现货

All lanes : Anti-Smarcc1 Antibody (C-term) at 1:1000 dilution Lane 1: C2C12 whole cell lysates Lane 2: C6 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded M. testis section using (Mouse) Smarcc1 Antibody (C-term). BD-PB3045 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of Hela cells stained with (Mouse) Smarcc1 Antibody (C-term). BD-PB3045 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

Western blot analysis of lysate from Hela cell line, using Smarcc1 Antibody (C-term). BD-PB3045 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.