BD-PB2933-MAD2L2 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

MAD2L2 Rabbit Polyclonal Antibody (C-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB2933

产品名称

MAD2L2 Rabbit Polyclonal Antibody (C-term)

别名

Mitotic spindle assembly checkpoint protein MAD2B, Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, REV7 homolog, hREV7, MAD2L2, MAD2B, REV7

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

Q9UI95

特异性

This MAD2L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 198-231 amino acids from the C-terminal region of human MAD2L2.

稀释度

IHC-P~~1:100~500;IF~~1:25;WB~~1:1000

预测分子量

24kDa

同种型

Rabbit Ig

背景介绍

Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.

组织表达

Ubiquitously expressed.

细胞定位

Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Chromosome. Note=Recruited to sites of chromosomal double-stranded breaks during G1 and S phase of the cell cycle

期货

现货

Immunohistochemical analysis of paraffin-embedded H. kidney section using MAD2L2 Antibody (C-term). BD-PB2933 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of Hela cells stained with MAD2L2 Antibody (C-term). BD-PB2933 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from Hela, K562 cell line (from left to right), using MAD2L2 Antibody (C-term). BD-PB2933 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.