BD-PB2924-DHFR Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

DHFR Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2924

产品名称

DHFR Rabbit Polyclonal Antibody (N-term)

别名

Dihydrofolate reductase, DHFR

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

P00374

特异性

This DHFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human DHFR.

稀释度

IHC-P~~1:100~500;WB~~1:1000

预测分子量

21kDa

同种型

Rabbit Ig

背景介绍

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.

组织表达

Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood Expression is higher in the adult brain than in the fetal brain

细胞定位

Mitochondrion {ECO:0000250|UniProtKB:P00375}. Cytoplasm {ECO:0000250|UniProtKB:P00375}

期货

现货

Immunohistochemical analysis of paraffin-embedded H. liver section using DHFR Antibody (N-term). BD-PB2924 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. brain section using DHFR Antibody (N-term). BD-PB2924 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from 293T, Hela, Jurkat, Y79 cell line , mouse spleen and rat kidney tissue lysate(from left to right), using DHFR Antibody (N-term). BD-PB2924 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.