BD-PB2911-NDUFB4 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

NDUFB4 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2911

产品名称

NDUFB4 Rabbit Polyclonal Antibody (N-term)

别名

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, CI-B15, NADH-ubiquinone oxidoreductase B15 subunit, NDUFB4

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

O95168

特异性

This NDUFB4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human NDUFB4.

稀释度

FC~~1:25;IF~~1:25;IHC-P~~1:100~500;WB~~1:1000

预测分子量

15kDa

同种型

Rabbit Ig

背景介绍

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

细胞定位

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side

期货

现货

Flow cytometric analysis of Hela cells using NDUFB4 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). BD-PB2911 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with NDUFB4 Antibody (N-term). BD-PB2911 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term). BD-PB2911 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2, PC-3, Ramos, U-251 MG cell line and human heart tissue lusyte(from left to right), using NDUFB4 Antibody (N-term). BD-PB2911 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.