All lanes : Anti-FXYD6 Antibody (C-term) at 1:1000 dilution Lane 1: Human cerebellum tissue lysate Lane 2: Human brain tissue lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.