BD-PB2911-NDUFB4 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

NDUFB4 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2911

产品名称

NDUFB4 Rabbit Polyclonal Antibody (N-term)

别名

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, CI-B15, NADH-ubiquinone oxidoreductase B15 subunit, NDUFB4

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

O95168

特异性

This NDUFB4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3-36 amino acids from the N-terminal region of human NDUFB4.

稀释度

WB 1:1000

预测分子量

15kDa

同种型

Rabbit Ig

背景介绍

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

细胞定位

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side

期货

现货

Flow cytometric analysis of Hela cells using NDUFB4 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). BD-PB2911 was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with NDUFB4 Antibody (N-term). BD-PB2911 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. skin section using NDUFB4 Antibody (N-term). BD-PB2911 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2, PC-3, Ramos, U-251 MG cell line and human heart tissue lusyte(from left to right), using NDUFB4 Antibody (N-term). BD-PB2911 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.