BD-PB2894-AK4 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

AK4 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2894

产品名称

AK4 Rabbit Polyclonal Antibody (Center)

别名

Adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255|HAMAP-Rule:MF_03170}, 27410 {ECO:0000255|HAMAP-Rule:MF_03170}, 2746 {ECO:0000255|HAMAP-Rule:MF_03170}, Adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170}, GTP:AMP phosphotransferase AK4 {ECO:0000255|HAMAP-Rule:MF_03170}, AK4 {ECO:0000255|HAMAP-Rule:MF_03170}

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Swissprot No.

P27144

特异性

This AK4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-153 amino acids from the Central region of human AK4.

稀释度

IF~~1:25;IHC-P~~1:100~500;WB~~1:1000

预测分子量

25kDa

同种型

Rabbit Ig

背景介绍

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.

组织表达

Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain

细胞定位

Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03170, ECO:0000269|PubMed:11485571, ECO:0000269|PubMed:19766732, ECO:0000269|PubMed:26980435}

期货

现货

Fluorescent image of HepG2 cells stained with AK4 Antibody (Center). BD-PB2894 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded M.kidney section using AK4 Antibody (Center). BD-PB2894 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.heart section using AK4 Antibody (Center). BD-PB2894 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.kidney section using AK4 Antibody (Center). BD-PB2894 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from A431, Hela, HepG2, MCF-7, Raji cell line and mouse kidney tissue lysate (from left to right), using AK4 Antibody (Center). BD-PB2894 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.