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PHB(10K13)Mouse Monoclonal Antibody

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产品基本信息

产品货号
BD-PB2878
产品名称
PHB(10K13)Mouse Monoclonal Antibody
别名
Prohibitin, PHB
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
Human Swissprot No.
P35232
特异性
This antibody is generated from a mice immunized with a recombinant protein between 1-205 amino acids from human.
稀释度
IHC-P~~1:100~500;WB~~1:1000
预测分子量
30kDa
同种型
IgG2b,k
背景介绍
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
组织表达
Widely expressed in different tissues.
细胞定位
Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane
期货
现货
纯化
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunohistochemical analysis of paraffin-embedded H. stomach section using PHB Antibody. BD-PB2878 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from 293,MCF-7,HepG2,mouse NIH/3T3 cell line and rat liver tissue (from left to right),using USF1 Antibody (Center) at 1:3000 dilution was used as the secondary antibody.Lysates at 35μg per lane.

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