BD-PB2861-R Cdk4 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

R Cdk4 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2861

产品名称

R Cdk4 Rabbit Polyclonal Antibody (C-term)

别名

Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, Cdk4

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

P35426

Rat Gene ID

NP_446045.1

特异性

This Rat Cdk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-303 amino acids from the C-terminal region of rat Cdk4.

稀释度

IHC-P~~1:100~500;IF~~1:25;WB~~1:1000

预测分子量

34kDa

同种型

Rabbit Ig

背景介绍

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

组织表达

Expressed in fetal and adult lung. Also expressed in brain, heart, liver, skeletal muscle and testes

细胞定位

Cytoplasm {ECO:0000250|UniProtKB:P11802}. Nucleus {ECO:0000250|UniProtKB:P11802}. Nucleus membrane {ECO:0000250|UniProtKB:P11802}. Note=Cytoplasmic when non-complexed Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus (By similarity). {ECO:0000250|UniProtKB:P11802}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunohistochemical analysis of paraffin-embedded R. brain section using R Cdk4 Antibody (C-term). BD-PB2861 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded rat small intestine section using Rat Cdk4 Antibody (C-term). BD-PB2861 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of MCF-7 cells stained with (Rat) Cdk4 Antibody (C-term). BD-PB2861 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Rat Cdk4 Antibody (C-term) western blot analysis in rat lung tissue lysates (35ug/lane).This demonstrates the rat Cdk4 antibody detected the rat Cdk4 protein (arrow).