Fluorescent image of NIH/3T3 cells stained with RNF166 Antibody (Center). BD-PB2838 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

RNF166 Antibody (Center) western blot analysis in 293,mouse NIH/3T3 cell line and testis tissue lysates (35ug/lane).This demonstrates the RNF166 antibody detected the RNF166 protein (arrow).