产品名称
HLA-DRB5 Rabbit Polyclonal Antibody (Center)
别名
HLA class II histocompatibility antigen, DR beta 5 chain, DR beta-5, DR2-beta-2, Dw2, MHC class II antigen DRB5, HLA-DRB5 {ECO:0000312|EMBL:CAI180791}
Human Gene ID
NP_002116.2
Human Swissprot No.
Q30154
特异性
This HLA-DRB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the Central region of human HLA-DRB5.
稀释度
WB~~1:1000;IHC-P~~1:100~500
背景介绍
HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
细胞定位
Cell membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:18305173, ECO:0000305}. Endoplasmic reticulum membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:18305173, ECO:0000305}. Golgi apparatus, trans- Golgi network membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:18305173, ECO:0000305}. Endosome membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:18305173, ECO:0000305}. Lysosome membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:18305173, ECO:0000305}. Late endosome membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:18305173, ECO:0000305}. Note=The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
