BD-PB2732-SGMS2 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

SGMS2 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2732

产品名称

SGMS2 Rabbit Polyclonal Antibody (C-term)

别名

Phosphatidylcholine:ceramide cholinephosphotransferase 2, Sphingomyelin synthase 2, SGMS2, SMS2

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001129729.1;NP_001129730.1;NP_689834.1

Human Swissprot No.

Q8NHU3

特异性

This SGMS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-365 amino acids from the C-terminal region of human SGMS2.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

42kDa

同种型

Rabbit Ig

背景介绍

Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene.

组织表达

Brain, heart, kidney, liver, muscle and stomach. Also expressed in a number of cell lines such as carcinoma HeLa cells, hepatoma Hep-G2 cells, and colon carcinoma Caco-2 cells

细胞定位

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Primarily localized at the plasma membrane with a small fraction at the Golgi apparatus.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

SGMS2 Antibody (C-term) western blot analysis in MCF-7,CEM,NCI-H460 cell line lysates (35ug/lane).This demonstrates the SGMS2 antibody detected the SGMS2 protein (arrow).

SGMS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SGMS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

SGMS2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.