BD-PB2725-ANKH Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ANKH Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2725

产品名称

ANKH Rabbit Polyclonal Antibody (C-term)

别名

Progressive ankylosis protein homolog, ANK, ANKH, KIAA1581

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_473368.1

Human Swissprot No.

Q9HCJ1

特异性

This ANKH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the C-terminal region of human ANKH.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

54kDa

同种型

Rabbit Ig

背景介绍

ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.

组织表达

Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells

细胞定位

Membrane; Multi-pass membrane protein

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-ANKH Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ANKH Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.

ANKH Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ANKH Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.