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GPI Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB2730
产品名称
GPI Rabbit Polyclonal Antibody (C-term)
别名
Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, AMF, Neuroleukin, NLK, Phosphoglucose isomerase, PGI, Phosphohexose isomerase, PHI, Sperm antigen 36, SA-36, GPI
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
Human Gene ID
NP_000166.2;NP_001171651.1
Human Swissprot No.
P06744
特异性
This GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI.
稀释度
WB 1:1000
预测分子量
63kDa
同种型
Rabbit Ig
背景介绍
GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
细胞定位
Cytoplasm. Secreted
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of lysates from Ramos cell line,mouse liver,rat liver tissue (from left to right),using GPI Antibody (C-term)at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

GPI Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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