产品名称
AGRIN Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O00468
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O00468/entry
Mouse Swissprot No.
A2ASQ1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/A2ASQ1
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P25304
免疫原
Synthesized peptide derived from human protein . at AA range: 1900-1980
特异性
AGRIN Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015],
组织表达
Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues.
细胞定位
[Isoform 1]: Secreted, extracellular space, extracellular matrix . Synaptic basal lamina at the neuromuscular junction. .; [Isoform 2]: Cell junction, synapse . Cell membrane ; Single-pass type II membrane protein .
信号通路
ECM-receptor interaction;
功能
function:Component of the basal lamina that causes the aggregation of acetylcholine receptors and acetylcholine-esterase on the surface of muscle fibers of the neuromuscular junction.,PTM:Contains heparan sulfate chains as well as N-linked and O-linked oligosaccharides.,similarity:Contains 1 NtA (N-terminal agrin) domain.,similarity:Contains 1 SEA domain.,similarity:Contains 2 laminin EGF-like domains.,similarity:Contains 3 laminin G-like domains.,similarity:Contains 4 EGF-like domains.,similarity:Contains 9 Kazal-like domains.,subcellular location:Synaptic basal lamina at the neuromuscular junction.,subunit:Binds to laminin.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
