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EYA4 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB2682
产品名称
EYA4 Rabbit Polyclonal Antibody (C-term)
别名
Eyes absent homolog 4, EYA4
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_004091.3;NP_742101.2;NP_742103.1
Human Swissprot No.
O95677
特异性
This EYA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the C-terminal region of human EYA4.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
70kDa
同种型
Rabbit Ig
背景介绍
EYA4 encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti.
组织表达
Highly expressed in heart and skeletal muscle.
细胞定位
Cytoplasm {ECO:0000250|UniProtKB:Q99502}. Nucleus {ECO:0000250|UniProtKB:Q99502}
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of EYA4 Antibody (C-term) in A2058 cell line lysates (35ug/lane). EYA4 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with EYA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EYA4 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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