BD-PB2658-EIF4A2 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

EIF4A2 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2658

产品名称

EIF4A2 Rabbit Polyclonal Antibody (C-term)

别名

Eukaryotic initiation factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA helicase eIF4A-2, EIF4A2, DDX2B, EIF4F

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001958.2

Human Swissprot No.

Q14240

特异性

This EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human EIF4A2.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

46kDa

同种型

Rabbit Ig

背景介绍

Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-EIF4A2 Antibody (C-term) at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of EIF4A2 Antibody (C-term) in K562 cell line lysates (35ug/lane). EIF4A2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EIF4A2 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.