BD-PB2656-DEK Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

DEK Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2656

产品名称

DEK Rabbit Polyclonal Antibody (C-term)

别名

Protein DEK, DEK

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001128181.1;NP_003463.1

Human Swissprot No.

P35659

特异性

This DEK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human DEK.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50;IF~~1:10~50

预测分子量

43kDa

同种型

Rabbit Ig

背景介绍

DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.

组织表达

Ubiquitous. Expressed at relatively high levels.

细胞定位

Nucleus. Note=Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of lysates from U-251 MG, PC-3 cell line (from left to right), using DEK Antibody (C-term)at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

DEK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DEK Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of DEK Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).