BD-PB2647-CLTA Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

CLTA Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2647

产品名称

CLTA Rabbit Polyclonal Antibody (Center)

别名

Clathrin light chain A, Lca, CLTA

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001070145.1;NP_001171689.1;NP_001171691.1;NP_001824.1;NP_009027.1

Human Swissprot No.

P09496

特异性

This CLTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the Central region of human CLTA.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

27kDa

同种型

Rabbit Ig

背景介绍

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.

细胞定位

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, spindle Note=Cytoplasmic face of coated pits and vesicles. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of CLTA Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CLTA (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CLTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of CLTA (arrow) using rabbit polyclonal CLTA Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTA gene.

CLTA Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.