BD-PB2646-EXOC5 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

EXOC5 Rabbit Polyclonal Antibody (C-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB2646

产品名称

EXOC5 Rabbit Polyclonal Antibody (C-term)

别名

Exocyst complex component 5, Exocyst complex component Sec10, hSec10, EXOC5, SEC10, SEC10L1

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_006535.1

Human Swissprot No.

O00471

特异性

This EXOC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-66 amino acids from the C-terminal region of human EXOC5.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

82kDa

同种型

Rabbit Ig

背景介绍

EXOC5 is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity.

组织表达

Ubiquitous..

细胞定位

Cytoplasm. Midbody. Note=Localization at the midbody requires the presence of RALA, EXOC2 and EXOC3

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-EXOC5 Antibody (C-term) at 1:1000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EXOC5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EXOC5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.