BD-PB2630-PSMB1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

PSMB1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2630

产品名称

PSMB1 Rabbit Polyclonal Antibody (C-term)

别名

Proteasome subunit beta type-1, Macropain subunit C5, Multicatalytic endopeptidase complex subunit C5, Proteasome component C5, Proteasome gamma chain, PSMB1, PSC5

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_002784.1

Human Swissprot No.

P20618

特异性

This PSMB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-241 amino acids from the C-terminal region of human PSMB1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50;FC~~1:10~50

预测分子量

26kDa

同种型

Rabbit Ig

背景介绍

PSMB1 is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

细胞定位

Cytoplasm. Nucleus

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-PSMB1 Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of PSMB1 Antibody (C-term) in mouse NIH-3T3 cell line(lane 1) and mouse bladder tissue(lane 2) lysates (35ug/lane). PSMB1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMB1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of PSMB1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

PSMB1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.