BD-PB2622-CTHRC1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

CTHRC1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2622

产品名称

CTHRC1 Rabbit Polyclonal Antibody (N-term)

别名

Collagen triple helix repeat-containing protein 1, Protein NMTC1, CTHRC1

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001243028.1;NP_612464.1

Human Swissprot No.

Q96CG8

特异性

This CTHRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-46 amino acids from the N-terminal region of human CTHRC1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

26kDa

同种型

Rabbit Ig

背景介绍

CTHRC1 may act as a negative regulator of collagen matrix deposition (By similarity).

组织表达

Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells.

细胞定位

Secreted, extracellular space, extracellular matrix

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-CTHRC1 Antibody (N-term) at 1:16000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human skin tissue reacted with CTHRC1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of CTHRC1 (arrow) using rabbit polyclonal CTHRC1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTHRC1 gene.

CTHRC1 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.