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PHYH Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB2618
产品名称
PHYH Rabbit Polyclonal Antibody (N-term)
别名
Phytanoyl-CoA dioxygenase, peroxisomal, Phytanic acid oxidase, Phytanoyl-CoA alpha-hydroxylase, PhyH, PHYH, PAHX
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001032626.1;NP_006205.1
Human Swissprot No.
O14832
特异性
This PHYH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-87 amino acids from the N-terminal region of human PHYH.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
39kDa
同种型
Rabbit Ig
背景介绍
PHYH2 catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.
组织表达
Expressed in liver, kidney, and T-cells, but not in spleen, brain, heart, lung and skeletal muscle
细胞定位
Peroxisome.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of PHYH Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). PHYH (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PHYH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PHYH (arrow) using rabbit polyclonal PHYH Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHYH gene.

PHYH Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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