BD-PB2575-AK3 Rabbit Polyclonal Antibody (C-term H38)-现货抗体产品库武汉枢密脑科学技术有限公司

AK3 Rabbit Polyclonal Antibody (C-term H38)

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产品基本信息

产品货号

BD-PB2575

产品名称

AK3 Rabbit Polyclonal Antibody (C-term H38)

别名

Adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255|HAMAP-Rule:MF_03170}, 27410 {ECO:0000255|HAMAP-Rule:MF_03170}, 2746 {ECO:0000255|HAMAP-Rule:MF_03170}, Adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170}, GTP:AMP phosphotransferase AK4 {ECO:0000255|HAMAP-Rule:MF_03170}, AK4 {ECO:0000255|HAMAP-Rule:MF_03170}

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001005353.1;NP_037542.1;NP_982289.1

Human Swissprot No.

P27144

特异性

This AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the C-terminal region of human AK3.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

25kDa

同种型

Rabbit Ig

背景介绍

AK3 is a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated.

组织表达

Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain

细胞定位

Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03170, ECO:0000269|PubMed:11485571, ECO:0000269|PubMed:19766732, ECO:0000269|PubMed:26980435}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

The anti-AK3 Pab is used in Western blot to detect AK3 in mouse kidney tissue lysate.

Western blot analysis of AK3 (arrow) using rabbit polyclonal AK3 Antibody (C-term H38). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2) .

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.