Cn|En

现货抗体产品库

PCK2 Rabbit Polyclonal Antibody (N-term)

产品详情 相关文献 产品问答 相关产品

产品基本信息

产品货号
BD-PB2572
产品名称
PCK2 Rabbit Polyclonal Antibody (N-term)
别名
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, PCK2, PEPCK2
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001018083.1;NP_004554.2
Human Swissprot No.
Q16822
特异性
This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human PCK2.
稀释度
WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50
预测分子量
71kDa
同种型
Rabbit Ig
背景介绍
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
细胞定位
Mitochondrion.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.

Confocal immunofluorescent analysis of PCK2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

相关文献

产品问答

相关产品

免责声明| 法律支持| 联系方式

市场:027-65023363   行政/人事:027-62439686   邮箱:marketing@brainvta.com  

销售总监:张经理  18995532642  华东区:陈经理 18013970337   华南区:王经理 13100653525   华中/西区:杨经理 18186518905   华北区:张经理 18893721749

地址:中国武汉东湖高新区光谷七路128号中科开物产业园1号楼

Copyright © 武汉枢密脑科学技术有限公司. All RIGHTS RESERVED.
鄂ICP备2021009124号 DIGITAL BY VTHINK