BD-PB2565-Endophilin B1 Rabbit Polyclonal Antibody (Y80)-现货抗体产品库武汉枢密脑科学技术有限公司

Endophilin B1 Rabbit Polyclonal Antibody (Y80)

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产品基本信息

产品货号

BD-PB2565

产品名称

Endophilin B1 Rabbit Polyclonal Antibody (Y80)

别名

Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1, SH3GLB1, KIAA0491

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001193580.1;NP_001193581.1;NP_001193582.1;NP_057093.1

Human Swissprot No.

Q9Y371

特异性

This Endophilin B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from human Endophilin B1.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

41kDa

同种型

Rabbit Ig

背景介绍

Endophilin B1 may be required for normal outer mitochondrial membrane dynamics. It is required for coatomer-mediated retrograde transport in certain cells. It may recruit other proteins to membranes with high curvature and may promote membrane fusion.

组织表达

Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes

细胞定位

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasmic vesicle, autophagosome membrane. Midbody. Note=Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140). {ECO:0000250, ECO:0000269|PubMed:17891140}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-Endophilin Antibody (Y80) at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-Endophilin B1 Antibody (Y80) in HepG2 cell line lysates (35ug/lane). SH3GLB1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.