BD-PB2551-Methionyl tRNA Synthetase Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Methionyl tRNA Synthetase Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2551

产品名称

Methionyl tRNA Synthetase Rabbit Polyclonal Antibody (C-term)

别名

Methionine--tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_612404.1

Human Swissprot No.

Q96GW9

特异性

This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

67kDa

同种型

Rabbit Ig

背景介绍

Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet <--> AMP + diphosphate + L-methionyl-tRNAMet The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met). This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.

细胞定位

Mitochondrion matrix.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-MARS2 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-MARS2 Antibody (C-term) in mouse testis tissue lysates (35ug/lane). MARS2(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.