BD-PB2545-CCK4 (PTK7) Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

CCK4 (PTK7) Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2545

产品名称

CCK4 (PTK7) Rabbit Polyclonal Antibody (N-term)

别名

Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7, PTK7, CCK4

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001257327.1;NP_002812.2;NP_690619.1;NP_690620.1;NP_690621.1

Human Swissprot No.

Q13308

特异性

This CCK4 (PTK7) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human CCK4 (PTK7).

稀释度

WB~~1:1000;FC~~1:10~50;IHC-P~~1:100~500

预测分子量

118kDa

同种型

Rabbit Ig

背景介绍

CCK4 may function as a cell adhesion molecule. Although it belongs to the insuline receptor subfamily of the Tyr protein kinases, it likely lacks the catalytic activity of a tyrosine kinase. It may be connected to the pathophysiology of colon carcinomas and/or may represent a tumor progression marker. This Type I membrane protein is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes, but weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle, and not in colon. It is also expressed in erythroleukemia cells.

组织表达

Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon

细胞定位

Membrane; Single- pass type I membrane protein. Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti-CCK4 (PTK7) Antibody (N-term) at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-CCK4 (PTK7) Antibody (N-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Caco2 whole cell lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-CCK4 (PTK7) Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Caco2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of CCK4 (arrow) using rabbit polyclonal CCK4 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) .

Flow cytometric analysis of NCI-H460 cells using CCK4 (PTK7) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.