产品名称
TRIB1 Rabbit Polyclonal Antibody (C-term)
别名
Tribbles homolog 1, TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1, TRIB1 (
HGNC:16891)
Human Gene ID
NP_079471.1
Human Swissprot No.
Q96RU8
特异性
This TRIB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1.
背景介绍
TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities.
Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.
组织表达
Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
