BD-PB2537-YES1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

YES1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2537

产品名称

YES1 Rabbit Polyclonal Antibody (N-term)

别名

Tyrosine-protein kinase Yes, Proto-oncogene c-Yes, p61-Yes, YES1, YES

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_005424.1

Human Swissprot No.

P07947

特异性

This YES1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human YES1.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

61kDa

同种型

Rabbit Ig

背景介绍

YES is a member of the Src family of non-receptor tyrosine kinases participating in a wide range of cellular signalling pathways. The gene that encodes YES is the cellular homolog of the Yamaguchi sarcoma virus oncogene. This gene lies in proximity to thymidylate synthase gene on chromosome 18, and a pseudogene of it has mapped to chromosome 22.

组织表达

Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis.

细胞定位

Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytosol Note=Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti-YES Antibody at 1:1000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.