BD-PB2529-PCSK2 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

PCSK2 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2529

产品名称

PCSK2 Rabbit Polyclonal Antibody (N-term)

别名

Neuroendocrine convertase 2, NEC 2, KEX2-like endoprotease 2, Prohormone convertase 2, Proprotein convertase 2, PC2, PCSK2, NEC2

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001188457.1;NP_001188458.1;NP_002585.2

Human Swissprot No.

P16519

特异性

This PCSK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the N-terminal region of human PCSK2.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50;FC~~1:10~50

预测分子量

71kDa

同种型

Rabbit Ig

背景介绍

PCSK2 belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This protein is a proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. The protein is also known to cleave proopiomelanocortin, proenkephalin, prodynorphin and proluteinizing-hormone-releasing hormone.

细胞定位

Cytoplasmic vesicle, secretory vesicle. Secreted. Note=Localized in the secretion granules

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-PCSK2 Antibody (N-term) at 1:1000 dilution + TT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of PCSK2 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). PCSK2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with PCSK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of PCSK2 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

PCSK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.