BD-PB2526-ACPP Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ACPP Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2526

产品名称

ACPP Rabbit Polyclonal Antibody (C-term)

别名

Prostatic acid phosphatase, PAP, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase, PAPf39, ACPP

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001090.2;NP_001127666.1

Human Swissprot No.

P15309

特异性

This ACPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-341 amino acids from the C-terminal region of human ACPP.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

45kDa

同种型

Rabbit Ig

背景介绍

ACPP is an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.

组织表达

Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues

细胞定位

[Isoform 1]: Secreted

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

All lanes : Anti-ACPP Antibody (C-term) at 1:1000 dilution Lane 1: Mouse prostate whole tissue lysate Lane 2: Rat prostate whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-ACPP Antibody (C-term) at 1:500 dilution Lane 1: PC-3 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: U-251MG whole cell lysate Lane 4: Mouse lung whole tissue lysate Lane 5: Mouse prostate whole tissue lysate Lane 5: Rat prostate whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-ACPP Antibody (C-term) in mouse lung tissue lysates (35ug/lane). ACPP(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACPP antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.