产品名称
PUM2 Rabbit Polyclonal Antibody (N-term)
别名
Pumilio homolog 2, Pumilio-2, PUM2, KIAA0235, PUMH2
Human Gene ID
NP_056132.1
Human Swissprot No.
Q8TB72
特异性
This PUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-162 amino acids from the N-terminal region of human PUM2.
稀释度
WB~~1:1000;IHC-P~~1:100~500
背景介绍
PUM2 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.
组织表达
Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested
细胞定位
Cytoplasm. Cytoplasmic granule. Cytoplasm, perinuclear region. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
