BD-PB2520-GCAT Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

GCAT Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2520

产品名称

GCAT Rabbit Polyclonal Antibody (Center)

别名

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001165161.1;NP_055106.1

Human Swissprot No.

O75600

特异性

This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

45kDa

同种型

Rabbit Ig

背景介绍

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

组织表达

Strongly expressed in heart, brain, liver and pancreas. Also found in lung.

细胞定位

Mitochondrion. Nucleus. Note=Translocates to the nucleus upon cold and osmotic stress

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of GCAT antibody (Center) in mouse liver tissue lysates (35ug/lane). GCAT (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.