BD-PB2519-CAPZA1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

CAPZA1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2519

产品名称

CAPZA1 Rabbit Polyclonal Antibody (N-term)

别名

F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_006126.1

Human Swissprot No.

P52907

特异性

This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CAPZA1.

稀释度

WB~~1:1000;IF~~1:10~50;FC~~1:10~50

预测分子量

33kDa

同种型

Rabbit Ig

背景介绍

CAPZA1 is a member of the F-actin capping protein alpha subunit family. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.

细胞定位

Cytoplasm, cytoskeleton.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

All lanes : Anti-CAPZA1 Antibody (N-term) at 1:4000 dilution Lane 1: A431 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of CAPZA1 antibody (N-term) in HL60 cell line lysates (35ug/lane). CAPZA1 (arrow) was detected using the purified Pab.

Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

CAPZA1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.