BD-PB2517-GPT Rabbit Polyclonal Antibody (N-term R133)-现货抗体产品库武汉枢密脑科学技术有限公司

GPT Rabbit Polyclonal Antibody (N-term R133)

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产品基本信息

产品货号

BD-PB2517

产品名称

GPT Rabbit Polyclonal Antibody (N-term R133)

别名

Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_005300.1

Human Swissprot No.

P24298

特异性

This GPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the N-terminal region of human GPT.

稀释度

IHC-P~~1:100;WB~~1:1000

预测分子量

55kDa

同种型

Rabbit Ig

背景介绍

GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.

组织表达

Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue

细胞定位

Cytoplasm.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunohistochemical analysis of paraffin-embedded H.liver section using GPT Antibody (N-term R133). BD-PB2517 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.kidney section using GPT Antibody (N-term R133). BD-PB2517 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

GPT Antibody (N-term R133) western blot analysis in U87-MG cell line , mouse liver ,and rat heart and liver tissue lysates (35ug/lane).This demonstrates the GPT antibody detected the GPT protein (arrow).