BD-PB2513-GOLPH3 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GOLPH3 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB2513

产品名称

GOLPH3 Rabbit Polyclonal Antibody (N-term)

别名

Golgi phosphoprotein 3, Coat protein GPP34, Mitochondrial DNA absence factor, MIDAS, GOLPH3, GPP34

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_071413.1

Human Swissprot No.

Q9H4A6

特异性

This GOLPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GOLPH3.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

34kDa

同种型

Rabbit Ig

背景介绍

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. This protein is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking.

组织表达

Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers

细胞定位

Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side Mitochondrion intermembrane space. Cell membrane Endosome. Note=Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of GOLPH3 antibody (N-term) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of hela cells using GOLPH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.