BD-PB2512-COG4 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

COG4 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB2512

产品名称

COG4 Rabbit Polyclonal Antibody (C-term)

别名

Conserved oligomeric Golgi complex subunit 4, COG complex subunit 4, Component of oligomeric Golgi complex 4, COG4

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001182068.1;NP_056201.2

Human Swissprot No.

Q9H9E3

特异性

This COG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-785 amino acids from the C-terminal region of human COG4.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

89kDa

同种型

Rabbit Ig

背景介绍

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG4.

细胞定位

Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Mosty cytosolic, with about 5% membrane-bound.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-COG4 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of COG4 Antibody (C-term) in A2058 cell line lysates (35ug/lane). COG4 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle with COG4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of jurkat cells using COG4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.