BD-PB2501-SMYD3 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

SMYD3 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2501

产品名称

SMYD3 Rabbit Polyclonal Antibody (Center)

别名

Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, SMYD3, ZMYND1, ZNFN3A1

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_001161212.1;NP_073580.1

Human Swissprot No.

Q9H7B4

特异性

This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3.

稀释度

WB~~1:1000;FC~~1:10~50

预测分子量

49kDa

同种型

Rabbit Ig

背景介绍

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.

组织表达

Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.

细胞定位

Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody (Center). BD-PB2501 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot analysis of SMYD3 antibody (Center) in CEM cell line lysates (35ug/lane). SMYD3 (arrow) was detected using the purified Pab.

SMYD3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.