BD-PB2463-PDK4 Rabbit Polyclonal Antibody (Center D98)-现货抗体产品库武汉枢密脑科学技术有限公司

PDK4 Rabbit Polyclonal Antibody (Center D98)

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产品基本信息

产品货号

BD-PB2463

产品名称

PDK4 Rabbit Polyclonal Antibody (Center D98)

别名

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_002603.1

Human Swissprot No.

Q16654

特异性

This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human PDK4.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

46kDa

同种型

Rabbit Ig

背景介绍

PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

组织表达

Ubiquitous; highest levels of expression in heart and skeletal muscle.

细胞定位

Mitochondrion matrix.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Anti-PDK4 Antibody (Center D98) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center D98) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of PDK4 Antibody (Center D98) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).