BD-PB2461-FPGS Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

FPGS Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB2461

产品名称

FPGS Rabbit Polyclonal Antibody (Center)

别名

Folylpolyglutamate synthase, mitochondrial, Folylpoly-gamma-glutamate synthetase, FPGS, Tetrahydrofolylpolyglutamate synthase, Tetrahydrofolate synthase, FPGS

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001018088.1;NP_004948.4

Human Swissprot No.

Q05932

特异性

This FPGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the Central region of human FPGS.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

65kDa

同种型

Rabbit Ig

背景介绍

FPGS is the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives.

细胞定位

[Isoform 1]: Mitochondrion inner membrane. Mitochondrion matrix

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-FPGS Antibody (Center) at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of FPGS Antibody (Center) in K562, A2058 cell line lysates (35ug/lane). FPGS (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of K562 cells using FPGS Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.